Histologists use various techniques to study stem cell heterogeneity, including:
Single-cell RNA sequencing (scRNA-seq): This technique allows for the analysis of gene expression at the single-cell level, revealing distinct subpopulations within a stem cell pool. Flow cytometry: By using specific markers, flow cytometry can isolate and quantify different stem cell subpopulations. Immunohistochemistry: This method uses antibodies to detect specific proteins, enabling the visualization of distinct stem cell types within tissue sections.
