Interpreting RT-PCR data involves: - Analyzing Ct Values: The cycle threshold (Ct) value is the point at which fluorescence exceeds the background level. Lower Ct values indicate higher initial amounts of target RNA. - Relative Quantification: Comparing the expression levels of the target gene to a reference or housekeeping gene to normalize the data. - Statistical Analysis: Using appropriate statistical methods to determine the significance of the observed changes in gene expression.
