Altered staining properties can occur due to several factors, including:
Fixation issues: Improper fixation can lead to poor preservation of tissue morphology, affecting dye uptake. pH levels: The pH of staining solutions can influence the binding of dyes to tissue components. Decalcification: Over-decalcification of bone tissues can reduce the staining intensity. Tissue processing: Issues during dehydration, clearing, and embedding can alter staining outcomes. Tissue type: Different tissues have inherent properties that can affect how well they take up stains.
