reverse transcription pcr (rt pcr)

What are the Limitations of RT-PCR?

Despite its many advantages, RT-PCR has some limitations:
- RNA Quality: The quality and integrity of RNA samples can significantly affect the results.
- Contamination: The process is susceptible to contamination, which can lead to inaccurate results.
- Technical Variability: Variations in the efficiency of reverse transcription and PCR amplification can affect the consistency of the results.

Frequently asked queries:

What is Reverse Transcription PCR (RT-PCR)?
How Does RT-PCR Work?
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What are the Limitations of RT-PCR?
How is RT-PCR Data Interpreted?
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