There are several types of probes used in RNA ISH, including:
- Oligonucleotide Probes: Short sequences of nucleotides that are complementary to the target RNA. - RNA Probes: Longer RNA sequences that are complementary to the target RNA, often generated through in vitro transcription. - Locked Nucleic Acid (LNA) Probes: Modified oligonucleotides that have increased binding affinity and stability. - Riboprobes: RNA probes labeled with radioactive or non-radioactive markers.
