PTEN can be detected in tissue samples using several histological techniques. Immunohistochemistry (IHC) is the most common method, utilizing specific antibodies to visualize PTEN protein expression. Additionally, fluorescence in situ hybridization (FISH) can be employed to detect PTEN gene deletions at the chromosomal level. Polymerase chain reaction (PCR) and sequencing techniques can also be used to identify PTEN mutations.
