Methylation analysis typically involves several steps: 1. DNA Extraction: High-quality DNA is extracted from the tissue sample. 2. Bisulfite Conversion: DNA is treated with sodium bisulfite, converting unmethylated cytosines to uracil while leaving methylated cytosines unchanged. 3. PCR Amplification: Specific regions of interest are amplified using PCR. 4. Sequencing or Array Hybridization: The PCR products are then sequenced or hybridized to arrays to identify methylation patterns.
