Manual counting is a meticulous process that generally involves the following steps: 1. Preparation of Slides: Tissue samples are fixed, embedded, sectioned, and stained using appropriate histological stains. 2. Microscopic Examination: The slide is placed under a microscope. Histologists use various magnifications to identify and count the cells or structures of interest. 3. Counting Methods: Different counting methods can be employed, such as counting within a defined area (e.g., a grid or a frame) or using a counting chamber. The counts are often recorded manually or with the help of a tally counter.
