Despite its advantages, cryobiology faces several challenges, such as:
Ice Crystal Formation: Even with cryoprotectants, ice crystals can form and damage cellular structures. Osmotic Stress: The introduction and removal of cryoprotectants can cause osmotic stress, leading to cell damage. Cost: The equipment and materials required for cryopreservation can be expensive. Standardization: Variability in cryopreservation protocols can affect the consistency of results.
