Sample Preparation: Tissues are collected, fixed, and embedded in paraffin or other media to preserve their structure. Sectioning: Thin slices of the tissue are cut using a microtome and placed on glass slides. Staining: Specific stains are applied to highlight different cellular components and structures. Common stains include Hematoxylin and Eosin (H&E), Masson's Trichrome, and Periodic Acid-Schiff (PAS). Microscopy: The prepared slides are examined under a microscope. Light microscopy, fluorescence microscopy, and electron microscopy are commonly used. Image Analysis: Digital images of the slides are captured and analyzed using specialized software to extract quantitative data.
