The process of histological staining typically involves several steps:
Fixation: Preserving the tissue to prevent degradation and maintain its structure. Formalin is a commonly used fixative. Dehydration: Removing water from the tissue using graded alcohol solutions. Embedding: Encasing the tissue in paraffin wax to provide support for thin sectioning. Sectioning: Cutting the tissue into very thin slices using a microtome. Staining: Applying the chosen stain to the tissue sections. Mounting: Placing the stained tissue sections on glass slides and covering them with a cover slip for microscopic examination.