The process of histochemical staining typically involves several steps:
Fixation: The tissue sample is preserved using a fixative like formalin to prevent degradation. Embedding: The fixed tissue is embedded in a medium, usually paraffin wax, to provide support during sectioning. Sectioning: Thin sections of the tissue are cut using a microtome and placed on microscope slides. Deparaffinization and Rehydration: The tissue sections are treated to remove paraffin and rehydrated through a series of alcohol and water solutions. Staining: The sections are stained using the chosen histochemical stain. Dehydration and Mounting: After staining, the sections are dehydrated and mounted with a coverslip for examination under a microscope.
