histochemical staining:

What are the Limitations of Histochemical Staining?

While histochemical staining is a powerful tool, it has some limitations:
Specificity: Some stains may not be entirely specific and can bind to multiple tissue components.
Subjectivity: Interpretation of stained tissues can be subjective and may vary between observers.
Technical Variability: Differences in staining protocols and conditions can lead to variability in results.
Sensitivity: Some stains may not detect low levels of the targeted component.

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