How is Glycosylation Detected in Histological Samples?
Glycosylation can be detected in histological samples using various techniques: - Lectin Histochemistry: Lectins are proteins that bind specifically to carbohydrate moieties. Using labeled lectins, one can visualize glycosylation patterns in tissue sections. - Immunohistochemistry (IHC): Antibodies specific to glycosylated epitopes can be used to detect glycoproteins in tissue samples. - Mass Spectrometry: Advanced techniques like mass spectrometry can be used to analyze glycosylation at a molecular level.
