GABA can be detected in tissue samples using various histological techniques:
Immunohistochemistry: This method uses antibodies specific to GABA to stain and visualize GABAergic neurons. In Situ Hybridization: This technique detects the mRNA of GABA-synthesizing enzymes, providing information on the cells actively producing GABA. Fluorescence Microscopy: Often combined with immunohistochemistry, this method helps in the detailed visualization of GABAergic neurons.
