How is F-12 Nutrient Mixture Used in Cell Culture?
F-12 Nutrient Mixture can be used alone or supplemented with other components such as fetal bovine serum (FBS) or specific growth factors to tailor it to the needs of particular cell types. The medium is typically sterilized through filtration and then used to culture cells in incubators set at specific temperature, humidity, and CO2 levels to maintain an optimal environment for cell growth.