Are There Any Limitations to Using F-12 Nutrient Mixture?
While F-12 is highly effective, it may not be suitable for all cell types. Some cells may require specific nutrients or conditions not provided by this medium. Additionally, because it is a synthetic medium, it may not fully replicate the complex biochemical environment of living tissues. Researchers often need to supplement F-12 with other factors or use it in conjunction with other media to achieve optimal results.