ER stress can be detected using various histological techniques: - Immunohistochemistry (IHC): This technique can be used to detect the presence and localization of ER stress markers like CHOP and BiP in tissue sections. - Western Blotting: Although not a histological technique per se, it can be used to quantify the levels of ER stress-related proteins in tissue extracts. - Electron Microscopy (EM): Provides ultrastructural details of the ER, showing dilation and morphological changes associated with ER stress.
