The process typically involves the following steps:
1. Isolation: Tissues or cells are isolated from an organism. 2. Disaggregation: Tissues are dissociated into single cells using enzymes like trypsin or mechanical methods. 3. Seeding: The cells are then placed in a culture medium, which provides necessary nutrients and growth factors. 4. Incubation: The culture is maintained under specific conditions, such as controlled temperature, pH, and CO2 levels.
