COPI activity is tightly regulated by several factors, including GTPases like ARF1 and various kinases and phosphatases that modify the coatomer complex and its associated proteins. The assembly and disassembly of the COPI coat are also regulated by the hydrolysis of GTP bound to ARF1, which triggers conformational changes that allow the coat to form or disassemble as necessary. Additionally, lipid composition of the membrane and various adaptor proteins play crucial roles in modulating COPI function.
