The process generally involves the following steps:
Deparaffinization: Removing paraffin wax from tissue sections if they are embedded in paraffin. Rehydration: Rehydrating the tissue sections through a series of alcohol washes. Application of the retrieval solution: Immersing the tissue sections in a retrieval solution and heating them to the appropriate temperature, often using a microwave or pressure cooker. Cooling and washing: Allowing the tissue sections to cool and then washing them to remove any residual chemicals.
