Cell line authentication is typically performed using several methods:
1. Short Tandem Repeat (STR) Profiling: This technique analyzes the unique patterns of DNA sequences to confirm the identity of the cell lines. 2. Karyotyping: This method involves examining the chromosomes of the cells to detect any abnormalities or misidentification. 3. Isoenzyme Analysis: This technique compares the enzyme patterns of the cell lines to known standards. 4. PCR-based Methods: Techniques such as species-specific PCR can identify contamination from other species.
