Autoradiography is used in various applications within histology and other biological sciences: - Mapping Metabolic Pathways: By using radiolabeled substrates, researchers can trace the pathways of metabolic processes and identify the location of metabolic activity within tissues. - Studying Gene Expression: Autoradiography can be used to detect mRNA levels by hybridizing radiolabeled nucleic acid probes to specific sequences, providing insights into gene expression patterns. - Tracking Cell Proliferation: Incorporating radiolabeled nucleotides, such as tritiated thymidine, into newly synthesized DNA allows visualization of cell division and proliferation.
