Preparation of agar based media involves several key steps:
1. Weighing and Mixing: The appropriate amount of agar powder is weighed and mixed with distilled water. Other ingredients, such as nutrients and selective agents, are also added. 2. Sterilization: The mixture is sterilized using an autoclave to eliminate any contaminating microorganisms. 3. Cooling and Pouring: After sterilization, the medium is cooled to about 45-50°C and then poured into sterile Petri dishes or culture tubes. 4. Solidification: The medium is allowed to solidify at room temperature before being stored or used for inoculation.
