The preparation of a negative control involves processing a tissue section in parallel with the test samples but omitting the primary antibody. The steps typically include: 1. Fixation and Embedding: The tissue is fixed and embedded in a medium like paraffin, similar to the test samples. 2. Sectioning: Sections are cut and placed on slides. 3. Blocking: Non-specific binding sites are blocked using a blocking solution. 4. Omission of Primary Antibody: Instead of applying the primary antibody, a diluent or buffer is used. 5. Secondary Antibody and Detection: The sections are then treated with the secondary antibody and detection reagents as per the protocol.
