Once a biopsy sample is collected, it undergoes several processing steps before being examined under a microscope:
Fixation: The tissue is preserved using chemical fixatives, such as formalin, to prevent degradation. Embedding: The fixed tissue is embedded in a solid medium, usually paraffin wax, to provide support for sectioning. Sectioning: Thin slices of the embedded tissue are cut using a microtome. Staining: The tissue sections are stained with dyes to highlight various cellular components. Common stains include Hematoxylin and Eosin (H&E). Microscopic Examination: The stained sections are examined under a microscope by a pathologist to identify any abnormalities.