Various advanced imaging techniques are employed to visualize 3D structures in histology. These include:
- Confocal Microscopy: This technique uses laser light to scan specimens and construct detailed 3D images by focusing on different planes within the tissue. - Multiphoton Microscopy: Similar to confocal microscopy, but uses longer wavelength light to penetrate deeper into tissues, making it ideal for imaging thick specimens. - Electron Microscopy: Provides high-resolution images of tissue architecture, although typically in 2D, serial sectioning and reconstruction can generate 3D models. - Optical Coherence Tomography (OCT): Uses light waves to capture micrometer-resolution, cross-sectional images of tissues, suitable for in vivo applications. - Micro-CT and MRI: These non-invasive imaging modalities are used to visualize the 3D structure of larger tissue samples and organs.
