The process of vector cloning involves several critical steps:
DNA Extraction: Obtain the DNA fragment of interest from a source organism. Digestion with Restriction Enzymes: Cut both the DNA fragment and the plasmid vector with compatible restriction enzymes. Ligation: Use DNA ligase to join the DNA fragment with the plasmid vector to create a recombinant DNA molecule. Transformation: Introduce the recombinant DNA into a host organism, often bacteria, for replication. Selection and Screening: Identify and isolate the host organisms that successfully incorporated the recombinant DNA using selection markers.
