Vacuum significantly enhances tissue processing by improving the efficiency and quality of each step:
- Fixation: Vacuum ensures the fixative solution penetrates tissues thoroughly, preserving cellular structures more effectively. - Dehydration and Clearing: The vacuum accelerates the removal of water and the introduction of clearing agents, reducing processing time and preventing tissue distortion. - Infiltration: By applying a vacuum, embedding agents like paraffin can infiltrate tissue samples more uniformly, preventing the formation of artifacts and ensuring better sectioning quality.
