The application of vacuum in histology is essential for several reasons:
1. Air Bubble Removal: Vacuum helps in removing air bubbles that can interfere with tissue infiltration and staining. 2. Enhanced Infiltration: A vacuum aids in the penetration of embedding mediums like paraffin into the tissue, ensuring uniform infiltration. 3. Improved Dehydration: During dehydration, a vacuum speeds up the removal of water and other solvents, reducing the processing time. 4. Electron Microscopy: In electron microscopy, a high vacuum is required to prevent scattering of electrons and to achieve high-resolution images.
