ISH involves several key steps. First, tissue samples are fixed and sectioned. Next, these sections are treated to allow probe penetration and hybridization. Labeled probes, which are complementary to the target nucleic acid sequence, are then applied to the samples. The hybridization between the probe and the target sequence occurs under specific conditions. After hybridization, excess probes are washed away, and the hybridized probes are detected using various methods, such as fluorescent or chromogenic labeling.
