The performance of biochemical tests involves several critical steps:
Sample Preparation: Tissue samples are fixed, usually in formalin, and embedded in paraffin. Thin sections are then cut and mounted onto slides. Deparaffinization and Rehydration: Slides are treated with xylene to remove paraffin and then rehydrated through a series of alcohols to water. Staining or Labeling: Specific reagents, antibodies, or probes are applied to the tissue sections to target the molecules of interest. Detection: Enzymatic reactions, fluorescent signals, or colorimetric changes are observed and documented using a microscope. Interpretation: Results are interpreted by comparing the staining patterns with known standards or controls.
