The staining process typically involves the following steps: 1. Fixation: The tissue sample is fixed using a chemical fixative to preserve its structure. 2. Application of Iodine: Iodine solution is applied to the tissue section, where it binds to polysaccharides such as glycogen. 3. Quinol Treatment: The tissue is then treated with quinol, which reduces the iodine-polysaccharide complex, resulting in a color change that highlights the stained structures under a microscope.
