How Does Cryosectioning Maintain Non-Denaturing Conditions?
Cryosectioning is a technique where tissues are rapidly frozen to preserve their native state. The frozen tissues are then sliced into thin sections using a cryostat. This method avoids the chemical fixation process, thus preserving the natural structure and molecular composition of the tissues. Cryosectioning is particularly useful for studying enzyme activities, receptor-ligand interactions, and other dynamic cellular processes.