Several techniques and methods can be employed to ensure non-denaturing conditions in histology:
1. Cryosectioning: This involves freezing tissues and cutting them into thin sections without using chemical fixatives that might alter protein conformation. 2. Mild Fixation: Using mild fixatives like paraformaldehyde at low concentrations can help preserve protein structures while immobilizing the tissues. 3. Detergent-Based Extraction: Non-ionic detergents like Triton X-100 can be used to permeabilize cells without denaturing proteins, making them accessible for further analysis.
