The process begins with the extraction of DNA from a tissue sample. The DNA is then denatured to create single-stranded templates. The ASO probes, which are labeled with a detectable marker (such as a radioactive isotope or fluorescent dye), are introduced to the denatured DNA. These probes will hybridize, or bind, only to their perfectly complementary sequences. After hybridization, the DNA is washed to remove any unbound probes. The presence of bound ASO probes indicates the presence of the specific allele being tested for.
