Rho GTPases function by interacting with a variety of effector proteins that influence diverse cellular processes. The activation of Rho GTPases leads to the formation of structures such as stress fibers, lamellipodia, and filopodia, which are critical for cellular movement and shape. The transition between the active and inactive states is regulated by Guanine nucleotide exchange factors (GEFs), GTPase-activating proteins (GAPs), and Guanine nucleotide dissociation inhibitors (GDIs).