Sample Preparation: This includes isolating RNA from the tissue of interest and ensuring its quality and integrity. Library Preparation: RNA is converted into complementary DNA (cDNA), which is then fragmented and tagged with sequencing adapters. Sequencing: The prepared cDNA library is sequenced using high-throughput sequencing technologies. Data Analysis: Sequencing data is processed to align reads to a reference genome, quantify gene expression, and identify differentially expressed genes.
