How Do Electron Microscopes Differ from Light Microscopes?
Electron microscopes differ from light microscopes in several ways:
Resolution: Electron microscopes have much higher resolution due to the shorter wavelength of electrons compared to visible light. Magnification: They provide significantly higher magnification, allowing for the detailed visualization of sub-cellular structures. Sample Preparation: Specimens for electron microscopy require more complex preparation, including fixation, dehydration, and embedding in resin. Imaging: TEM produces two-dimensional images by transmitting electrons through a thin specimen, while SEM provides three-dimensional images by scanning the surface with a focused beam of electrons.