Several factors can lead to poor visibility, including:
Improper Staining: Incorrect staining techniques or the use of outdated or poor-quality dyes can result in weak or uneven staining, making tissue structures hard to distinguish. Inadequate Fixation: Poorly fixed tissues may degrade, leading to a loss of structural integrity and clarity. Substandard Microscopy: Using low-resolution microscopes or incorrect lens magnifications can affect visibility. Tissue Processing Errors: Inadequate dehydration, clearing, or embedding of tissues can result in poorly prepared samples.