Minimizing tissue shrinkage involves optimizing various steps in tissue processing:
1. Fixation: Using fixatives that cause minimal shrinkage, such as formalin at lower concentrations, can help reduce the extent of shrinkage. 2. Dehydration: Gradual dehydration with alcohols of increasing concentrations can help reduce shrinkage. 3. Clearing and Embedding: Using clearing agents and embedding media that cause minimal shrinkage can also be beneficial. Alternatives to xylene, such as citrus-based agents, may reduce shrinkage. 4. Temperature Control: Maintaining optimal temperatures during processing can also help minimize shrinkage.
