To minimize the occurrence of folds in histological sections, several best practices can be followed:
Ensure proper embedding with the tissue oriented correctly and fully supported by paraffin. Use a sharp and well-maintained microtome blade to achieve smooth and even cuts. Optimize the cutting angle and pressure to match the tissue type. Use a water bath at the appropriate temperature to float and stretch the sections without causing artifacts. Carefully transfer sections from the water bath to the slide to avoid creating wrinkles.
