How Can Autofluorescence Be Minimized or Controlled?
Several strategies can be employed to minimize the impact of autofluorescence:
Chemical Quenching: Using chemical agents like Sudan Black B can help reduce autofluorescence. Spectral Unmixing: Advanced imaging techniques can separate the autofluorescent signal from the specific fluorescent label. Use of Longer Wavelengths: Employing fluorophores that emit at longer wavelengths can sometimes bypass the autofluorescent signals. Pre-treatment of Tissue: Tissue treatments such as UV irradiation can help reduce autofluorescence.
