The development of tissue models involves several key steps:
Cell Selection: The choice of cells is critical, as they must represent the target tissue accurately. These can be primary cells extracted from tissues or stem cells that can differentiate into the desired cell type. Scaffold Design: Scaffolds provide a three-dimensional structure for cells to grow and organize. They can be made from natural materials like collagen or synthetic polymers like polyethylene glycol. Bioreactors: Bioreactors are used to provide a controlled environment for tissue development. They supply nutrients, remove waste, and can apply mechanical stimuli to mimic the natural environment of the tissue. Bioactive Molecules: Growth factors, cytokines, and other bioactive molecules are often added to guide cell differentiation and tissue formation.
