Identification of stem cells in histological samples involves several techniques:
1. Immunohistochemistry (IHC): This technique uses antibodies to detect specific stem cell markers such as Oct4, Sox2, and Nanog in tissue sections. 2. Flow Cytometry: While not strictly a histological method, it is often used alongside to sort and identify stem cells based on their surface markers. 3. In situ Hybridization: This method detects specific nucleic acid sequences within tissue sections, helping identify gene expression patterns associated with stem cells.