Soluble factors can be studied using various techniques:
- Immunohistochemistry (IHC): This technique uses antibodies to detect and visualize specific soluble factors within tissue sections. It provides information on the spatial distribution and relative abundance of these molecules.
- Enzyme-Linked Immunosorbent Assay (ELISA): A quantitative method used to measure the concentration of soluble factors in biological samples such as blood, serum, or tissue extracts.
- Western Blotting: This technique detects specific proteins in a sample by separating them based on size and using antibodies for identification.
- RNA Sequencing: This advanced method analyzes the expression levels of genes that encode for soluble factors, providing insights into their regulation under different conditions.
