The preparation of serial sections involves several steps: 1. Fixation: The tissue is preserved using a fixative, such as formalin, to prevent degradation. 2. Embedding: The fixed tissue is embedded in a medium, usually paraffin wax, to provide support during slicing. 3. Sectioning: Using a microtome, the tissue is cut into thin sections, typically 3-5 micrometers thick. 4. Mounting: Each section is mounted onto a separate glass slide. 5. Staining: The sections are stained using appropriate histological stains to highlight different cellular components.
