Establishing primary cell cultures involves several steps:
Tissue Extraction: Fresh tissue is obtained from an organism. Tissue Disaggregation: The tissue is enzymatically or mechanically broken down to release individual cells. Seeding: Disaggregated cells are placed into culture dishes with nutrient-rich media to support growth. Incubation: Cells are incubated under optimal conditions (temperature, CO2 concentration) to promote proliferation.
