How are Plant Pathologies Diagnosed Histologically?
Histological diagnosis of plant pathologies involves several steps: 1. Sample Collection: Diseased plant tissues are collected for examination. 2. Fixation: Tissues are fixed using chemicals such as formaldehyde to preserve their structure. 3. Sectioning: Fixed tissues are embedded in paraffin wax and sectioned into thin slices using a microtome. 4. Staining: Sections are stained with dyes like Hematoxylin and Eosin (H&E) to highlight different tissue components. 5. Microscopic Examination: Stained sections are examined under a microscope to identify histological changes, such as cell wall thickening, necrosis, and hypertrophy.
