Metabolic indicators can be identified using various histological techniques:
Histochemical Staining Histochemical staining methods, such as Periodic Acid-Schiff (PAS) for carbohydrates and Oil Red O for lipids, are commonly used to visualize specific metabolic indicators within tissues.
Immunohistochemistry Immunohistochemistry (IHC) involves the use of antibodies to detect specific enzymes or proteins associated with metabolic processes. This method provides precise localization of metabolic indicators within tissues.
Electron Microscopy Electron microscopy offers high-resolution imaging of cellular structures, allowing for detailed examination of mitochondria and other organelles involved in metabolism. It is particularly useful for studying mitochondrial density and structure.